Micro bca protein assay kit 23235 was purchased from. Pierce bca protein assay kit, sufficient reagents for 250 testtube or 2500 microplate assays. Compatibility chart for bca kit the amount listed is the maximum amount of material allowed in the protein sample without causing a noticeable interference, when 20 ul protein sample is used for bca assay as described in the standard assay. The thermo scientific pierce bca protein assay kit is a detergentcompatible formulation based on bicinchoninic acid bca for the.
This modification of the bicinchoninic acid bca protein assay is suitable for dilute protein samples 0. Trademarks that are the property of other companies. The pierce micro bca protein assay reagent kit is a detergentcompatible bicinchoninic acid formulation for the colorimetric detection and quantitation of total protein. Pierce bca protein assays have a unique advantage over the coomassie dyebased assays bradfordthey are compatible with samples that contain up to 5% surfactants detergents, and are affected much less by protein compositional. Thermo scientific pierce 660 nm protein assay overview highlights typical response curves 14 bca based protein assays 15 chemistry of the bca protein assay 15 advantages of the bca protein assay 16 disadvantages of the bca protein assay 17 bca, reducing agent compatible 18 bca protein assay 19 micro bca protein assay 20. Pierce bca protein assay reagent a bca protein assay reagent a liquid. Value when the 660nm assay is run using the ionic detergent compatibility reagent idcr, part no.
Pierce microplate bca protein assay kit fisher scientific. Oct 22, 2007 the bca protein assay kit from thermo scientific pierce product no. Pierce bca protein assay kit thermo fisher scientific. The first step is the chelation of copper with protein in an alkaline environment to form a light blue complex. Used in more labs than any other detergentcompatible protein assay, pierce bca reagents provide accurate determination of protein concentration with most sample types. If the total protein concentration in the samples is high 2,000. The 384well, flat bottom, nontreated, transparent, sterile polystyrene plates cat. See thermo scientific halt protease and phosphatase inhibitors on pages 4446 4. Using the matrix platemate 2x2 to automate the bca protein. Compared to most dyebinding methods, the bca assay is affected much less by protein compositional differences, providing greater protein to protein uniformity. Assays were performed according to the standard testtube procedure using 100. This protocol makes use of thermo scientifics bca protein assay in a 96well tissue culture plate format to measure the amount of protein in small volumes of sample.
Measuring protein concentration using the bca protein assay with. This was done by analyzing the precision, accuracy, linearity and range of the method. Thermo scientific pierce protein assay technical handbook. This reducing agentcompatible rac version of the thermo scientific pierce bca protein assay provides accurate determination of protein concentration in samples that contain dithiothreitol dtt, 2mercaptoethanol bme, tcep and other disulfide reducing agents. Thermo scientific slidealyzer mini dialysis units 6. Determination of dissociation constant of lysozyme binding to tio2 or wo3 lysozyme was dissolved in water to give concentrations of 0, 5, 10, 20, 30, 40, 50, 60, and 70. Thermo scientific pierce 660 nm protein assay overview highlights typical response curves 14 bcabased protein assays 15 chemistry of the bca protein assay 15 advantages of the bca protein assay 16 disadvantages of the bca protein assay 17 bca, reducing agent compatible 18 bca protein assay 19 micro bca protein assay 20. The bca protein assay is suitable for measuring protein concentration in the range of 0.
This reassessment of bca assay is important to avoid misestimating biases in bioconjugation processes. The pierce bca assay can be used to assess yields in whole cell lysates and affinitycolumn fractions, as well as to monitor protein contamination in industrial applications. Safety data sheet chandlergilbert community college. The thermo scientific pierce bca protein assay kit is a twocomponent, highprecision, detergentcompatible assay reagent set to measure a562nm total protein concentration compared to a protein standard. Protein yield comparison of two bacterial cell lysis reagents. Using the matrix platemate 2x2 to automate the bca protein assay. Convenientcomplete set containing seven readytouse dilutions universalrecognized.
Studies of the bca assay for determination of total protein in allergen extracts title swedish abstract the aim of this thesis has been to evaluate and to optimize the bca protein assay for quantification of total protein. Per reagents are thermo scientific pierce cell lysis reagents. Langes handbook of chemistry, 14 th edition, dean, j. The working reagent, based on bicinchoninic acid bca, is used for the colorimetric detection of protein and is diluted to a working range. Pierce bca protein assay kit from thermo scientific. Thermo scientific pierce bca protein assay kit fisher. Samples prepared in memper reagent can be dialyzed if the buffer contains detergent e. The micro bca protein assay kit is a specialized version of the popular pierce bca protein assay for determining the protein concentration of dilute samples. Thermo scientific pierce bca protein assay kit reducing. I have been using a pierce bca protein assay kit thermo scientific and diluting my samples saliva. Thermo scientific pierce bca protein assay kit bca protein. Application note determination of protein concentrations with varioskantm using bca protein assay this paper describes the use of thermos varioskan spectrophotometer and spectrofluorometer for protein concentration determinations using bca protein assay and photometric detection. This kit was previously called bca protein quantification kit.
In this study, we have successfully compared manual and automated methods of performing the bicinchoninic acid bca protein assay. Measuring protein concentration using the bca protein assay. Thermo scientific pierce cell lysis technical handbook. The pierce 660 nm protein assay has a greater linear range of 252,000. The intensity of the blue complex is proportional to the amount of protein in the sample. Compared to most dyebinding methods, the bca assay is affected much less by protein compositional differences, providing greater proteintoprotein uniformity. Feb 14, 2014 the principle of lowry assay, biuret assay, and bicinconinich bca assay protein assays duration. We recommend the use of gloves, lab coats and eye protection. Bicinchoninic acid bca protein assay, gbiosciences vwr. Pierce bca protein assays have a unique advantage over the coomassie dyebased assays bradfordthey are compatible with samples that contain up to 5% surfactants detergents, and are affected much less by protein compositional differences, providing greater protein to protein uniformity. The thermo scientific micro bca protein assay kit is a detergentcompatible bicinchoninic acid formulation for the colorimetric detection and quantitation of. Studies of the bca assay for determination of total protein.
Correction in bicinchoninic acid bca absorbance assay to. Incompatible substances amount compatible buffer systems nacetylglucosamine 10 mm in pbs, ph 7. The bca protein assay is a popular method for colorimetric detection and quantitation of total protein. Reducing agent compatible, sufficient reagents to assay 250 samples or standards of 25l each. The bicinchoninic acid bca protein assay is a highly sensitive colorimetric assay that is compatible with detergent solubilized protein solutionsaccurately quantifying total protein concentration is a key to most experiments and is often necessary for isolation, separation, and analysis of. The thermo scientific pierce bca protein assay kit is a detergent compatible formulation based on bicinchoninic acid bca for the. Smith at the pierce chemical company, now part of thermo fisher scientific, is a biochemical assay for determining the total concentration of protein in a solution 0. Bca protein assay kit from thermo scientific pierce. The thermo scientific pierce bca protein assay is a detergentcompatible formulation based on bicinchoninic acid bca for the colorimetric detection and quantitation of total protein. An adaptation of pierces bca protein assay reagent kit product no. Thermo scientific pierce bca protein assay kit from thermo. Thermo scientific pierce protein assay technical handbook version 2. The bicinchoninic acid assay bca assay, also known as the smith assay, after its inventor, paul k.
Bca protein assay kit, sufficient reagents for 250 test tube or 2,500 microplate assays. The principle of lowry assay, biuret assay, and bicinconinich bca assay protein assays duration. Pierce microplate bca protein assay kit reducing agent compatible, sufficient reagents for. The calibration is almost the same for day to day but i still recommend calibrating for each assay. Competitive binding to cuprous ions of protein and bca in the. The bicinchoninic acid bca protein assay is a highly sensitive colorimetric assay that is compatible with detergent solubilized protein solutionsaccurately quantifying total protein concentration is a key to most experiments and is often necessary for isolation, separation, and analysis of proteins by biochemical methods. Thermo scientific pierce bsa protein assay standards are highquality reference samples for generating accurate standard curves and calibration controls in total protein assays. Pierce bca protein assay kit is a twocomponent, highprecision, detergentcompatible protein assay for determination of protein concentration. Thermo scientific pierce 660 nm protein assay reagent.
Special 3component version of our popular bca reagents to measure total protein concentration of dilute protein solutions 0. Setting up and running miniprotean tgx precast gels duration. Measuring the protein concentration of samples is essential for many protein assays, such as western blotting or zymography. Pierce bovine serum albumin standard prediluted set. Reagents bicinchoninic acid solution, catalog number b9643 reagent a is a 1,000 ml. Bca protein assay reagent bicinchoninic acid duration. Protocol the following are instructions for the microplate assay format of the pierce bca protein assay kit thermo scientific cat. Micro bca protein assay kit 23235 was purchased from thermo. Mixing together the three micro bca reagents results in a working solution that is sufficiently concentrated to measure protein when mixed with an equal volume of sample. Jun 19, 2018 bca protein assay reagent bicinchoninic acid duration. The pierce bca protein assay kit was purchased from thermo scientific waltham, ma and the working reagent wr was prepared as instructed in the users manual. The bca protein assay kit from thermo scientific pierce product no. Acid bca protein assay is a highly sensitive colorimetric assay that is compatible with detergent solubilized protein solutions. Biomedical and biological sciences 61,877 views 24.
Additional information and online ordering used in more labs than any other detergentcompatible protein assay, pierce bca reagents provide accurate determination of protein concentration with most sample types. An adaptation of the thermo scientific bca protein assay kit product no. Bca protein assay reagent bicinchoninic acid youtube. The assay is much more convenient than having to make up your own solutions for a biuret or lowry assay. The accuracy of gelatin quantification is further improved by reducing the bca assay incubation time to approximately 20 min, compared with the recommended 30 min. The bicinchoninic acid assay bca, also referred to as the smiths assay, is a biochemical assay designed by paul smith in 1985. Prepare pierce bovine serum albumin bsa standards 2,000. Micro bca protein assay kit thermo fisher scientific. Bicinchoninic acid protein assay kit bca1 technical bulletin. Protein assay versus the thermo scientific pierce 660 nm protein assay.
Protein assay compatibility table thermo fisher scientific. The pierce bca protein assay is a detergentcompatible formulation based on bicinchoninic acid bca for the colorimetric detection and quantitation of total protein. Bicinchoninic acid bca assay in low volume sciencedirect. Bca protein assay reagent bicinchoninic acid from thermo. The thermo scientific pierce bca protein assay kit is a detergentcompatible formulation based on bicinchoninic acid bca for the colorimetric detection and quantitation of total protein. Greentoblue a562nm, precise, detergentcompatible assay reagent to measure total protein concentration vs. Piercea protein assay kit bc thermo fisher scientific. Micro bca protein assay kit 23235 was purchased from thermo scientific.
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